Clones were isolated and gene fragments were sequenced . subsequent gene sequence database were also establishen to manage the identified gene information . the purified cdna fragments were used as probes for the gene chips and arrayed upon microscopic slides by cartesian microarrayer 本研究利用rd ? pcr ( restrictiondisplay - pcr ， rd ? pcr )技术，从酵母细胞中分离出大量cdna片段，克隆和构建了cdna片段文库，进一步将单基因片段纯化出来，建立了相应的数据库对所有信息进行管理。
